• 专利附录
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    • 专利摘要:
    The present disclosure provides a method for improving the germination rate of Macleaya cordata seeds, which includes the following steps: treating the Macleaya cordata seeds with an exogenous reagent, and carrying out cultivation and 5 germination on a cultivation medium after sand-storage and lamination, wherein the exogenous reagent is a mixed solution of concentrated hydrochloric acid, cytokinin, gibberellin and polyethylene glycol in sequence. The method provided by the present disclosure can remarkably promote germination of the Macleaya cordata seeds, the germination rate is improved to 75-80% and is improved by nearly 3 times, artificial 10 cultivation of the Macleaya cordata seeds is facilitated, and the cultivation cost is greatly saved. 14
    公开号:NL2027306A
    申请号:NL2027306
    申请日:2021-01-13
    公开日:2021-10-25
    发明作者:Gao Zhen；Zhang Zixuan；Luo Lina；Xiang Zengxu；Zhang Chengcai
    申请人:Univ Nanjing Agricultural；
    IPC主号:
    专利说明:

    METHOD FOR IMPROVING GERMINATION RATE OFMACLEAYA CORDATA SEEDS
    TECHNICAL FIELD The disclosure pertains to the technical field of virtual reality, and specifically pertains to an interaction control method and an interaction control device for virtual reality.
    BACKGROUD Macleaya cordata is a perennial erect herb, and belongs to Papaveraceae plants. The whole plant is extremely poisonous, and the whole herb can be used as medicine, but cannot be taken orally. It is often used for treating rheumatism and bone diseases, traumatic injuries and swelling, mosquito bites, skin itches and acne, etc. It has a remarkable therapeutic effect. At present, Macleaya cordata is in great need in clinical medical treatment and pesticide production. Macleaya cordata mainly grows in hills, mountain forests, grass clusters and bushes with an altitude of 150-830 meters. It likes a warm and humid growing environment, is cold-resistant and drought-resistant, and has no high requirements on soil, but it is better to be planted in fertile soil. The main reproductive mode of Macleaya cordata is seed propagation, but the biggest problem in seed propagation is that the germination rate of Macleaya cordata seeds under natural conditions is not high, which is between 20-35%, and thus it is difficult to carry out large-scale artificial cultivation. In recent years, it has been found that sanguinarine contained in the pods and leaves of Macleaya cordata is a natural substitute for antibiotic drugs, which makes the value of Macleaya cordata be multiplied and the demand of it exceeds supply. Macleayva cordata is accelerating the formation of a new industry in planting medicinal materials. Therefore, for solving the shortage of wild resources of Macleaya cordata, it is of great significance to provide a method for improving the germination rate of Macleaya cordata seeds. 1
    SUMMARY In view of this, an objective of the present disclosure is to provide a method for improving the germination rate of Macleaya cordata seeds, which can significantly improve the germination rate of Macleaya cordata seeds and facilitate large-scale artificial cultivation of Macleaya cordata.
    To solve the aforementioned technical problem, the present disclosure provides the following technical solution.
    Provided is a method for improving the germination rate of Macleaya cordata seeds, which includes the following steps: treating the Macleaya cordata seeds with an exogenous reagent, and carrying out cultivation and germination on a cultivation medium after sand-storage and lamination; wherein the exogenous reagent is a mixed solution of concentrated hydrochloric acid, cytokinin, gibberellin and polyethylene glycol in sequence.
    Preferably, the time for the treatment with concentrated hydrochloric acid is 15-25 min.
    Preferably, the cytokinin is 6-BA with a concentration of 2.0 mg/L, and the time for soaking withitis 1.5-3 h.
    Preferably, the mixed solution of gibberellin and polyethylene glycol is specifically a mixed aqueous solution of 300-500 mg/L of gibberellin and 40-50 mg/L of polyethylene glycol, and the soaking time is 24-36 h.
    Preferably, the time for sand-storage and lamination is 20-30 d.
    Preferably, the cultivation medium is a mixture of river sand, nutrient soil, coconut chaff and vermiculite.
    More preferably, the volume ratio of the river sand, nutrient soil, coconut husk and vermiculite in the mixture is 1-1.5:1.8-3.2:0.5-1:0.5-1.
    Preferably, the temperature for the cultivation and germination is 18-30°C and the humidity for the cultivation and germination is 60-90%.
    Preferably, the Macleava cordata seeds are sown after cultivation and germination, sufficient decomposed farmyard manure needs to be applied before sowing, the decomposed farmyard manure and the river sand are mixed uniformly at a ratio of 4:1, and the amount of the decomposed farmyard manure as applied is 1,200-1,500 kg/667 m*.
    2
    Beneficial effects of the present disclosure: The method of the present disclosure adopts an exogenous reagent to treat the Macleaya cordata seeds, and promotes the germination of the seeds by utilizing the exogenous reagent. It can effectively break the dormancy of the seeds and improve the germination rate of the seeds.
    At the same time, by treatment with sand-storage and lamination for 20-30 d, under the condition that the optimum cultivation conditions and the optimum medium are selected, the germination rate of the Macleaya cordata seeds is improved to 75-80%, which is nearly 3 times higher than that of the existing Macleaya cordata seeds, and is beneficial to realize large-scale artificial cultivation of Macleaya cordata.
    DETAILED DESCRIPTION The present disclosure provides a method for improving the germination rate of Macleaya cordata seeds, which includes the following steps: treating the Macleaya cordata seeds with an exogenous reagent, and carrying out cultivation and germination on a cultivation medium after sand-storage and lamination; wherein the exogenous reagent is a mixed solution of concentrated hydrochloric acid, cytokinin, gibberellin and polyethylene glycol in sequence.
    The Macleava cordata seeds employed in the present disclosure are preferably fresh Macleaya cordata seeds as collected. In the present disclosure, before the treatment of the Macleaya cordata seeds with the exogenous reagent, preferably it also includes cleaning the Macleaya cordata seeds. The cleaning is specifically cleaning the collected Macleaya cordata seeds with warm water, and sorting mature seeds with plump grains. The temperature of the warm water is preferably 35-40°C, and more preferably 37-39°C.
    The Macleaya cordata seeds of the present disclosure are treated with the exogenous reagent. In the present disclosure, the Macleava cordata seeds are firstly treated with concentrated hydrochloric acid; the time for the treatment with concentrated hydrochloric acid is 15-25 min, and more preferably 18-22 min. In the present disclosure, the concentrated hydrochloric acid can soften the seed coat of the Macleaya cordata seeds and enhance the water permeability and air permeability of the seeds, thereby promoting the germination of the Macleaya cordata seeds. In the present disclosure, there is no special requirement on the mass fraction of concentrated hydrochloric acid, and concentrated hydrochloric acid with a 3 conventional mass fraction in the art can be used.
    In the present disclosure, the seeds treated with concentrated hydrochloric acid are soaked with cytokinin. In the present disclosure, the cytokinin is preferably 6-BA; the concentration of 6-BA is preferably 0.5-2.5 mg/L, more preferably 1.5-2.3 mg/L, and most preferably 2.0 mg/L; OS and the soaking time is preferably 1.5-3 h, and more preferably 2.0-2.5 h. The cytokinin of the present disclosure promotes the germination of the Macleaya cordata seeds by promoting the cell division and elongation of the Macleava cordata seeds.
    In the present disclosure, the Macleava cordata seeds are then soaked with the mixed solution of gibberellin and polyethylene glycol. The mixed solution of gibberellin and polyethylene glycol of the present disclosure is preferably a mixed aqueous solution of 300-500 mg/L gibberellin and 40-50 mg/L polyethylene glycol, and more preferably a mixed aqueous solution of 350-450 mg/L gibberellin and 43-46 mg/L polyethylene glycol. The time for seed soaking is preferably 24-36 h, and more preferably 28-30 h. Through rational proportioning, the mixed solution of gibberellin and polyethylene glycol of the present disclosure can promote cell growth, thereby improving the germination rate of the Macleaya cordata seeds.
    In the present disclosure, after the Macleaya cordata seeds are treated with the exogenous reagent, preferably it needs to mix the seeds with river sand according to a ratio of 1:(3.5-4), and then the mixture 1s covered with the river sand for the treatment of sand-storage and lamination. The depth of the overlapping river sand is preferably 2.5-4.5 cm, and more preferably 3-4 cm. The time for the sand-storage and lamination is preferably 20-30 d, and more preferably 25-28 d.
    In the present disclosure, the seeds treated by sand-storage and lamination are preferably germinated in the cultivation medium. The cultivation medium of the present disclosure is preferably a mixed medium consisting of river sand, nutrient soil, coconut chaff and vermiculite.
    The volume ratio in the mixed medium is preferably river sand: nutrient soil: coconut chaff: vermiculite = 1-1.5:1.8-3.2:0.5-1:0.5-1, and more preferably river sand: nutrient soil: coconut chaff: vermiculite = 1:2:1:1.
    In order to determine the optimum cultivation medium, the Macleava cordata seeds treated by the exogenous reagent and sand-storage and lamination are respectively sown on four cultivation media of A: nutrient soil; B: vermiculite: nutrient soil: peat: garden soil = 1:2:1:1; C: river sand: nutrient soil: coconut chaff: vermiculite = 1:2:1:1; and D: vermiculite: garden soil = 1:1. The following table can be obtained by screening the optimum cultivation medium through 4 determination of the germination rate of the Macleava cordata seeds: Table 1 Effect of different medium ratios on the germination rate of the Macleava cordata seeds Serial Number Medium ratio (volume ratio) Germination rate/% A Nutritional soil 75.3 B Vermiculite: nutritional soil: 80.5 peat: garden soil = 1:2:1:1 C C: river sand: nutritional soil: 92.4 coconut chaff: vermiculite = 1:2:1:1 D D: vermiculite: garden soil = 78.4 1:1 It can be seen from Table 1 that, different medium ratios have different effects on the germination rate of the Macleaya cordata seeds, wherein the germination rate of the Macleava cordata seeds is the highest when river sand: nutrient soil: coconut chaff: vermiculite = 1:2:1:1 is used as the cultivation medium. That is, river sand: nutrient soil: coconut chaff: vermiculite =1:2:1:1 is the optimum cultivation medium. In the present disclosure, the germination conditions of the Macleaya cordata seeds are preferably a temperature of 18-30°C and humidity of 60-90%.
    In order to determine the optimum germination conditions of the Maecleaya cordata seeds, on the basis of the selected optimum cultivation medium (river sand: nutrient soil: coconut chaff: vermiculite = 1:2:1:1), under the condition that different temperatures of 18°C, 24°C and 30°C; and different humidity of 60%, 75% and 90% are set, the following table can be obtained by determining the germination rate of the Macleaya cordata seeds: Table 2 Effects of different temperatures and humidity on the germination rate of the Macleaya cordata seeds Serial Number Temperature (°C) Humidity (%) Germination rate (%) 1 18 60 89.3 5
    2 18 75 85.7 3 18 90 67.5 4 24 60 93.5 24 75 90.2 6 24 90 70.6 7 30 60 75.4 8 30 75 72.6 9 30 90 60.2 It can be seen from Table 2 that different temperature and humidity conditions have different effects on the germination rate of the Macleaya cordata seeds, and the germination rate of the Macleaya cordata seeds is the highest, reaching 93.5% under the cultivation and 5 germination condition of the temperature of 24°C and the humidity of 60%.
    In the present disclosure, preferably the cultivated and germinated Macleaya cordata seeds are sown, and preferably, sufficient decomposed farmyard manure also needs to be applied before sowing. In the present disclosure, preferably the decomposed farmyard manure and river sand are mixed for fertilization. The purpose of uniformly mixing the farmyard manure and the river sand in the present disclosure is to increase the air permeability of the soil, thereby promoting the germination of the seeds. The decomposed farmyard manure and the river sand are preferably mixed uniformly at a ratio of (3.5-4.5):1, and more preferably at a ratio of 4:1; the amount of the decomposed farmyard manure as applied is preferably 1,200-1,500 kg/667 m’.
    The experimental result shows that: in the present disclosure, the Macleaya cordata seeds treated with the exogenous reagent are sown on the optimum cultivation medium for cultivation, and the germination rate of the Macleava cordata seeds is improved to 75-80%, which is nearly 3 times higher than that of the existing Macleaya cordata seeds.
    In order to make the objectives, technical solutions and advantages of the present disclosure more apparent, the present disclosure will be described in detail hereafter in connection with examples, but they cannot be understood as limiting the claimed scope of the present disclosure.
    Example 1 6
    (1) The collected Macleaya cordata seeds were cleaned with warm water, mature seeds with plump grains were sorted, treated with concentrated hydrochloric acid for 25 min, and then rinsed with clear water; (2) after the treatment of step (1) was completed, the Macleaya cordata seeds were soaked 9S with 2.0 mg/L of the cytokinin 6-BA for 3 h; (3) after the treatment of step (2) was completed, the Macleaya cordata seeds were rinsed with water for 30 min, and soaked with the mixed solution of 500 mg/L of gibberellin and 50 mg/L of polyethylene glycol for 24 h, and then the seeds were taken out and air-dried to remove the moisture on the surface of the seeds; then the Macleaya cordata seeds were uniformly mixed with river sand at a ratio of 1:4, the mixture was put into a mesh bag, the mesh bag was covered with the river sand at a depth of 4.5 cm, and then subjected to the treatment of sand storage for 20 d; (4) in the middle of March of the next year, the Macleaya cordata seeds treated with sand storage were cultured and germinated in an illumination incubator (at the temperature of 24°C and the humidity of 60%), and the germination of the Aacleaya cordata seeds during 1-8 weeks of culture was observed.
    Example 2 (1) The collected Macleava cordata seeds were cleaned with warm water, mature seeds with plump grains were sorted, treated with concentrated hydrochloric acid for 15 min, and then rinsed with clear water; (2) after the treatment of step (1) was completed, the Macleava cordata seeds were soaked with 2.0 mg/L of the cytokinin 6-BA for 1.5 h; (3) after the treatment of step (2) was completed, the Macleaya cordata seeds were rinsed with water for 20 min, and soaked with the mixed solution of 300 mg/L of gibberellin and 40 mg/L of polyethylene glycol for 36 h, and then the seeds were taken out and air-dried to remove the moisture on the surface of the seeds; then the Macleava cordata seeds were uniformly mixed with river sand at a ratio of 1:4, the mixture was put into a mesh bag, the mesh bag was covered with the river sand at a depth of 2.5 cm, and then subjected to the treatment of sand storage for 30 d; (4) in the middle of March of the next year, the Macleava cordata seeds treated with sand 7 storage were cultured and germinated in an illumination incubator (at the temperature of 24°C and the humidity of 60%), and the germination of the Macleava cordata seeds during 1-8 weeks of culture was observed.
    Example 3 (1) The collected Macleaya cordata seeds were cleaned with warm water, mature seeds with plump grains were sorted, treated with concentrated hydrochloric acid for 22 min, and then rinsed with clear water; (2) after the treatment of step (1) was completed, the Macleaya cordata seeds were soaked with 2.0 mg/L of the cytokinin 6-BA for 2.5 h; (3) after the treatment of step (2) was completed, the Macleaya cordata seeds were rinsed with water for 30 min, and soaked with the mixed solution of 400 mg/L of gibberellin and 45 mg/L of polyethylene glycol for 30 h, and then the seeds were taken out and air-dried to remove the moisture on the surface of the seeds; then the Macleava cordata seeds were uniformly mixed with river sand at a ratio of 1:4, the mixture was put into a mesh bag, the mesh bag was covered with the river sand at a depth of 3.5 cm, and then subjected to the treatment of sand storage for 28 d; (4) in the middle of March of the next year, the Macleaya cordata seeds treated with sand storage were cultured and germinated in an illumination incubator (at the temperature of 24°C and the humidity of 60%), and the germination of the Macleava cordata seeds during 1-8 weeks of culture was observed.
    Example 4 The operation steps were similar to those in Example 3, and the only difference was that in step (4), the Macleaya cordata seeds treated with sand storage were sown in a greenhouse (at the temperature of 18-25°C and the humidity of 60-90%) for culture and germination, and the germination of the Macleava cordata seeds during 1-8 weeks of culture was observed and recorded.
    Example 5 The operation steps were similar to those in Example 3, and the only difference was that in step (4), the Macleava cordata seeds treated with sand storage were cultured and germinated in an outdoor field, and the germination of the Macleaya cordata seeds during 1-8 weeks of culture was observed and recorded.
    8
    The following table could be obtained by determining the germination rate of Macleava cordata under different cultivation manners in Examples 3-5: Table 3 Germination rate of the Macleaya cordata seeds under different cultivation manners Melod Time Example 3 Example 4 Example 5 One week 0 0 0 Two weeks 0 0 0 Three weeks 16.85% 10.48% 5.74% Four weeks 54.58% 46.45% 32.46% Five weeks 60.60% 55.26% 46.73% Six weeks 70.30% 60.38% 55.56% Seven weeks 88.56% 74.56% 65.28% Eight weeks 93.58% 85.46% 75.36% It could be seen that the Macleaya cordata seeds treated with the exogenous reagent of the present disclosure were cultivated in the optimum cultivation medium after the treatment of sand-storage and lamination, and the germination rate of the Macleaya cordata seeds could be remarkably improved whether the cultivation and germination was conducted in an illumination incubator, a greenhouse or an outdoor field with a complicated environment.
    Comparative Example 1 The operation steps were the same as those in Example 3, and the only difference was that in step (1), the Macleaya cordata seeds were not treated with concentrated hydrochloric acid, and the germination of the Macleava cordata seeds in seven weeks was observed and recorded. Comparative Example 2 The operation steps were the same as those in Example 3, and the only difference was that in step (2), the Macleaya cordata seeds were not soaked with the cytokinin 6-BA, and the germination of the Macleava cordata seeds in seven weeks was observed and recorded. Comparative Example 3 The operation steps were the same as those in Example 3, and the only difference was that 9 in step (3), the Macleaya cordata seeds were not soaked with the mixed solution of gibberellin and polyethylene glycol, and the germination of the Macleaya cordata seeds in seven weeks was observed and recorded. Comparative Example 4 The operation steps were the same as those in Example 3, and the only difference was that in step (3), the Macleaya cordata seeds were first soaked with the mixed solution of gibberellin and polyethylene glycol, and then soaked with the cytokinin 6-BA, and the germination of the Macleaya cordata seeds in seven weeks was observed and recorded. The following table could be obtained by determining the germination rate of Macleava cordata in Example 3 and Comparative Examples 1-4: Table 4 Effects of different exogenous reagents on the germination rate of the Macleaya cordata seeds. Groups Example 3 Comparative | Comparative | Comparative | Comparative Example 1 Example 2 Example 3 Example 4 Germination rate of one week (%) Germination 88.56 72.30 67.56 45.37 8247 rate of seven weeks (%) It could be seen from Table 4 that different exogenous reagents had different effects on the germination rate of the Macleaya cordata seeds, and both the types and use sequences of the exogenous reagents would affect the germination rate of the Macleava cordata seeds. By adopting the types and sequences of the exogenous reagents of the present disclosure, the germination rate of the Macleaya cordata seeds could be remarkably improved, reaching
    88.56%. As can be seen from the aforementioned examples, the method of the present disclosure can promote the germination of the Macleaya cordata seeds and effectively break the dormancy of the seeds. Compared with the germination rate of the Macleaya cordata seeds under natural conditions, the germination rate under outdoor field conditions is increased to 75-80%, which is convenient for artificial cultivation of Macleaya cordata and greatly reduces the reproductive 10 cost of Macleaya cordata.
    The above description is only embodiments of the present disclosure and is not intended to limit the patent scope of the present disclosure. Any equivalent structure or equivalent flow transformation made by using the contents of the specification of the present disclosure, or direct D or indirect uses in other related technical fields, are similarly included in the claimed patent scope of the present disclosure. 11
    权利要求:
    Claims (10)
    [1]
    A method for improving the germination rate of Macleaya cordata seeds, comprising treating the Macleaya cordata seeds with an exogenous reagent, and performing cultivation and germination on a cultivation medium after sand storage and lamination; wherein the exogenous reagent is a mixed solution of concentrated hydrochloric acid, cytokinin, gibberellin and polyethylene glycol in sequence.
    [2]
    The method according to claim 1, wherein the time for the treatment with concentrated hydrochloric acid is 15-25 min.
    [3]
    The method of claim 1, wherein the cytokinin is 6-BA at a concentration of 2.0 mg/L, and the time for soaking is 1.5-3 hours.
    [4]
    The method of claim 1, wherein the mixed solution of gibberellin and polyethylene glycol is especially an aqueous mixed solution of 300-500 mg/L of gibberellin and 40-50 mg/L of polyethylene glycol.
    [5]
    The method of claim 4, wherein the time for soaking with the mixed solution of gibberellin and polyethylene glycol is 24-36 hours.
    [6]
    The method of claim 1, wherein the time for sand storage and lamination is 20-30 d.
    [7]
    The method of claim 1, wherein the cultivation medium is a mixture of river sand, nutrient medium, coconut husk and vermiculite.
    [8]
    A method according to claim 7, wherein the volume ratio of the river sand, the nutrient medium, coconut bark and vermiculite in the mixture is 1-1.5:1.8-3.2:0.5-1:0.5-1.
    [9]
    The method of claim 1, wherein the cultivation and germination temperature is 18-30°C and the cultivation and germination humidity is 60-90%.
    [10]
    The method of claim 1, wherein the Macleaya cordata seeds are sown after cultivation and germination, sufficiently digested farmyard manure is applied before sowing, the digested farmyard manure and river sand are uniformly mixed at a ratio of 4:1, and the amount of the digested manure as applied 1,200-1,500 kg/667 m is.
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    同族专利:
    公开号 | 公开日
    CN111316785A|2020-06-23|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    JPS6157802B2|1979-06-07|1986-12-09|Kojin Kk|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    CN202010242506.9A|CN111316785A|2020-03-31|2020-03-31|Method for improving germination rate of macleaya cordata seeds|
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